Protective Effects of Annona Squamosa on Cardiovascular Congenital Anomalies in Streptozotocin Induced Diabetic Albino Rats

Protective Effects of Annona Squamosa on Cardiovascular Congenital Anomalies in Streptozotocin Induced Diabetic Albino Rats

Authors

  • Mah Jabeen Muneera
  • Qamar Ashfaq Ahmad
  • Mohammad Tahir

DOI:

https://doi.org/10.21649/akemu.v18i2.390

Abstract

Objective:  To evaluate the protective effects of aque-ous leave extract of Annona squamosa on cardio vas-cular congenital anomalies in diabetic albino rats.

Methods:  It was a randomized control study, conduc-ted in the Department of Anatomy, University of Heal-th Sciences Lahore Pakistan, from May 2007 to 08. 18 adult albino pregnant rats with initial weight of 200 – 250 gm were randomly selected and divided into three groups; Group A served as control, whereas diabetes was induced by single injection of streptozotocin (45 mg / kg) on 8th gestational day in groups B and C. The diabetes was confirmed by checking blood glucose level from tail vein of the rats. Group C was addition-ally treated with the aqueous extract of Annona squa-mosa leaf (350 mg / kg / day) from 10th to 20th day of pregnancy. Fetuses were obtained by sacrificing the animals on 20th gestational day. Fetuses were eutha-nized, decapitated and fixed in 10% neutral buffered formalin; thoracic cavity was opened, heart and great vessels were observed thoroughly under stereomicro-scope. Heart was removed and processed for histolo-gical examination; serial sections, 5 µm thick, were obtained, stained with H & E and examined with the light microscope. The design and protocol of the study was approved by the ethical committee of the institute.

Results:  18.2% fetuses from B group showed transpo-sition of great vessels and atrial septal defect. Mean thickness of interventricular septum, in group B was significantly increased (881.0 ± 117.4 µ) as compared to that in groups A (487.7 ± 141.0 µ) and C (558.8 ± 86.4 µ, p < 0.05) indicating marked septal hypertro-phy. Valvular defects were also observed in fetuses from group B. Such anomalies were not discernable in group C fetuses.

Conclusion:  It is concluded that use of Annona squa-mosa leave extract alleviates diabetes induced cardiac malformations.

Key Words:  Diabetes mellitus, Annona squamosa, cardiac anomalies.

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How to Cite

Muneera, M. J., Ahmad, Q. A., & Tahir, M. (2013). Protective Effects of Annona Squamosa on Cardiovascular Congenital Anomalies in Streptozotocin Induced Diabetic Albino Rats. Annals of King Edward Medical University, 18(2), 137. https://doi.org/10.21649/akemu.v18i2.390

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