Role of Ascorbic Acid on Chromium Induced Changes in Rat Liver – A Morphometric Analysis.

Abstract

This study was conducted with an aim of forming a morphometric base line for normal and treated liver tissue, establishing the comparative damage done by Chromium (Cr) alone and in combination with Ascorbic Acid in a time related sequence. A study involving 120 rats, randomly divided into 4 groups — A, B, C & D of 30 animals each. Every group subdivided in 3 subgroups each, according to treatment time i.e. 2,4 and 8 weeks. Group B was administered Cr. solution, intraperitoneally, group C was given Cr. solution ip (intraperitoneally) and Ascorbic Acid orally, group D received Ascorbic Acid alone. Control group A was administered distilled water intraperitoneally. Chromium toxicity exhibited as thickened liver capsule, dilated central vein, and increased hepatocytic dimensions, which got statistically significant with advance in treatment. Comparable group receiving both Cr. and Ascorbic Acid showed slightly milder form of the same changes. Chromium hepatoxicity in low doses is thus established thus confirming its potential health hazards. Role of Ascorbic Acid in Cr. poisoning needs to be exposed further in terms of administration route and dosage.