Microbiological Diagnosis of Osteoarticular Infections and their Antibiogram

Abstract

Background: Osteoarticular infections in adults and children are a significant cause of elevated morbidity and may lead to restrictive mobility of various stages. Objective: Isolation and determination of the occurrence, pathomorphological and antibiotic susceptibility patterns of isolated microorganisms from the patients with osteoarticular infections. Methods: This research work was carried out at the Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore (UOL). Pus specimens (n = 120) were gathered from patients of osteoarticular infections. Bacterial isolates were purified and identified biochemically. Antibiotic resistance of the bacterial isolates was investigated by the criterion set by Clinical and Laboratory Standards Institute (CLSI). All experiments were run in triplicate using randomized study design. The mean, standard error and standard deviation values were determined using SPSS (v. 23.0). Results: Out of 120 samples, 111 isolated samples (93%) were tested positive for total viable count. The isolated bacterial species were observed to be Streptococcus pyogenes, Staphylococcus hemolyticus, S. aureus, Pseudomonas aeruginosa, Escherichia coli, Micrococcus sp., Serratia sp., Klebsiella sp., Enterobacter sp., and Proteus sp. Antibiogram results also yielded S. pyogenes and S. hemolyticus to be erythromycin resistant, while S. aureus was vancomycin resistant. E. coli and Klebsiella sp. were found to be resistant to tobramycin while Proteus and Enterobacter sp. were both sensitive to it. Conclusion: P. aeruginosa, E. coli, and S. aureus were prevalent in all groups of age, while Micrococcus and Serratia sp. were common in 16-55 years. Patient hygiene, immune health and the course of medications are all factors that should be kept in consideration while treating the disease.