Histo-Pathological Study: Skin Cancer Varieties Due to a Compound of Coal Tar & of Vinegar

Abstract

Objectives:  To see skin cancer varieties due to coal tar & vinegar, the present studies, using mice as a model, evaluated histo-pathological and certain biochemical changes induced in skin by repeated topical application of their compounds, DMBA and TPA.

Materials & Methods:  Hundred mice distributed into four groups of twenty five mice each were utilized. Two separate control groups were maintained. In the first experimental group, DMBA alone and in the second experimental group, TPA after single application of DMBA were applied on the skin of mice. Histo-pathological characterizations and cellular DNA, RNA and proteins molecules concentrations’ estimation of the lesion’s cells were evaluated through the comparative optical density change of the absorbance for DNA, RNA and Proteins molecules of different samples with that of their control throughout this twenty weeks study.

Results:  It was observed that the repeated application of DMBA alone and repeated application of TPA after single application of DMBA produced both epidermal and dermal tumours like papilloma, dysplasia, keratoacanthoma, squamous cell carcinoma in situ, extensive squamous cell carcinoma, and fibrosarcoma. The malignant varieties of skin tumors were 30 % more with the repeated application of DMBA alone than that by repeated application of TPA after single application of DMBA. The statistical analysis of cellular DNA, RNA and proteins concentrations estimates were found comparatively significant in experimental groups.

Conclusion:  The contact of DMBA (a compound of coal tar) can act as initiator as well as promoter for skin tumourigenisis.

Abbreviations:

¤ DMBA (Dimethyl Benz anthracene) is derived from anthracene i.e. poly-aromatic hydrocarbon found in commonly used coal tar.¤¤ TPA (tetradecanyl phorbol acetate) is derived from acetate that is salt or ester of ethanoic acid manufactured by oxidation of ethanol and commonly used for production of vinegar.